Bestimmung von Serumalbumin und Serumglobulinen in Altersabhängigkeit beim Hund

Determination of serum albumin and serum globulins in dependency of
age in dogs The age dependency of the parameters total protein,
albumin, alpha-1-globulins, alpha-2-globulins, beta-globulins,
gamma-globulins and the albumin-globulin-ratio in dogs was examined
in this study. Therefore 406 blood samples from clinically healthy
dogs were taken. The dogs ranged in age from six weeks to 16 years
and were distributed in nine groups of age. The protein fractions
were determined by two cellulose acetate electrophoresis gadgets.
Therefore an automatic and a semiautomatic gadget were used.
Additionally, the bromcresol green method was used for the
quantitative determination of albumin. Regarding the age dependency
of the examined parameters the results of the used gadgets
correspond to each other. The problem of imprecision of cellulose
acetate electrophoresis which is described in literature was also
noticed in this study; the results of the automatic gadget were
more exact. For each of the abovely mentioned parameters a
significant age dependency could be proofed. Total protein
increased continuously and significantly during the whole life. The
mean value increased correspondingly from 45.5 +/- 1.55 g/l (0 up
to 6 weeks old animals) up to 66.1 +/- 4,40 g/l (more than 10 years
old animals). The values of albumin determined by the bromcresol
green method increased statistically demonstrable until the age of
one to two years (mean values: 30.0 +/- 1.81 g/l (0 up to 6 weeks
old animals), 36.8 +/- 3.15 g/l (more than 1 and up to 2 years old
animals)). Afterwards no significant changes could be seen.
Concerning the values of relative albumin, a lifelong, significant
decrease resulted from measuring with the automatic electrophoresis
gadget; the mean value decreased from 67.2 +/- 2.67 % to 56.2 +/-
3.20 %. By the following, the results and the mean values of the
globulin fractions, determined by the automatic gadget, are listed:
The absolute values of the alpha-1- globulin fraction decreased
statistically provable from puppy age (0 up to 6 weeks old animals:
4.1 +/- 0.37 g/l) to the end of the first year of life (more than 6
and up to 12 months old animals: 3.4 +/- 0.65 g/l). The relative
values of this fraction showed the same attitude from puppy age
(8.9 +/- 0.57 %) up to the group of the one to two years old
animals (5.1 +/- 1.11 % ). The absolute values of alpha-2-globulins
increased significantly from the youngest group (0 up to 6 weeks
old animals: 2.8 +/- 0.39 g/l) up to the group of the one to two
years old animals (4.9 +/- 1.34 g/l) and from the group of the five
to eight years old animals (4.9 +/- 1.34 g/l) up to the oldest
group (more than 10 years old animals: 5.7 +/- 1.18 g/l). Between
the 2nd and the 8th year of life no significant differences could
be found. The relative values of alpha-2-globulins increased
significantly between the youngest (6.2 +/- 0.82 %) and the oldest
group (8.6 +/- 1.61 % ). For the beta- globulin fraction a lifelong
significant increase of the absolute (from 6.3 +/- 0.99 g/l to 13.5
+/- 1,61 g/l) and relative values (from 13.8 +/- 1.99 % to 20.3 +/-
2.06 %) were found. However, for the age from four months up to one
year of life no statistically relevant increase could be seen. The
relative and the absolute values of gamma-globulins increased
significantly from puppy age (1.8 +/- 0.27 g/l, 3.9 +/- 0.64 %) to
the group of one to two years old animals (6.2 +/- 1.51, 9.8 +/-
2.05 %). The albumin- globulin- ratio showed a lifelong significant
decrease qualifying that between the 6th month and the 5th year of
life no statistically relevant changes were provable. The mean
value decreased from 2.1 +/- 0.27 (0 up to 6 weeks old animals) to
1.3 +/- 0.18 (more than 10 years old animals). To investigate the
influence of the different growth and ageing process and the
different life expectancy of small, medium, large and giant breeds
on the course of the examined parameters, groups of weight were
compared in different groups of age. But distributing the dogs to
groups of weight could not analyze this problem expressively.