Zeta-Ketten Expression bei Patienten mit SCCHN und dendritische Zellen als Stimulatoren für T-Zellen In Vitro

Introduction The zeta-chain as an important component of the T cell
receptor (TCR) is involved in the transduction of intracellular
signals and is therefore critical for T cell activation and
subsequent induction of an anti-tumor response. In patients with
squamous cell carcinoma of the head and neck (SCCHN) several
mechanisms of immune suppression have been described. The
expression of the TCR -chain of both peripheral blood lymphocytes
(PBLs) and tumor infiltrating lymphocytes (TILs) has been analysed
in patients with SCCHN and correlated to clinical data. Methods TIL
and PBL from 47 patients with SCCHN were obtained. To determine
expression of the zeta-chain in both PBLs and TILs, double
immunostaining with mAbs and flow cytometric analysis was
performed. The technique combined an intracellular staining with a
surface staining. Results As compared to healthy controls (n=23),
zeta-chain expression was significantly reduced (p