Bestimmung der Vitamin D- Aktivität von calcinogenen Pflanzen im Wachteleischalentest

Determination of vitamin D- activity of calcinogenic plants in the
Japanese quail eggshell test In this studie the biological activity
of various preparations of the calcinogenic plants Solanum
glaucophyllum and Trisetum flavescens is analysed by means of the
Japanese quail eggshell test. As main parameters for the evaluation
of the biological activity served on the one hand the amount of
eggshell dryweight excreted per animal per day as a indicator for
the calcium excretion via the eggshell, on the other hand the serum
alkaline phosphatase and the content of realitve boneash.
Furthermore, additional egg-, bone- and blood parameters were
determined. In the two test series which lasted 21 days each
vitamin D depleted quails (Coturnix coturnix japonica) were
utilized as test animals. In both test series three vitamin
D3–standard ratios (100 IU, 200 IU und 400 IU/kg diet) and the
metabolite 1,25(OH)2D3 in different concentrations were used. The
preparations of Solanum glaucophyllum to be tested was a
watersoluble raw extract, an enriched pure extract, a hydrolyzed
preparation and preparations of stems and leaves and a preparation
of Trisetum flavescens. The influence of glucosidase on the test
parameters was analyzed too. The existing linear relationship
between vitamin D3-concentration in the diet of the ovendry mass of
the eggshells per animal and day forms the basis of the biological
activity of the preparations examined.