podcast.de
Anmeldung
Anmeldung Registrierung
Auto Hell Dunkel
Erweiterte Suche
  1. Startseite
  2. Podcasts
  3. Medizin - Open Access LMU - Teil 15/22 Podcast
  4. Dependence of TIMP-1 plasma levels on preanalytical specimen handling
Background: Tissue inhibitor of metalloproteinases-1 (TIMP-1) in
blood might be a helpful biomarker in various diseases. However,
various authors report that TIMP-1 is dependent on preanalytical
procedures. Our study was performed to determine how storage
conditions and time to centrifugation influence TIMP-1. Materials
and Methods: Twenty-six blood specimens were collected from each of
20 volunteers. Two specimens from each person were
centrifuged/measured within 1 h after venipuncture and frozen at
-80 degrees C. They were thawed once or twice within 72 h. Eight
specimens were stored at 20 degrees C in daylight, 8 at 20 degrees
C covered and 8 at 4 C in daylight. Four of each of these 8
specimens were mixed once a day until centrifugation. A mixed and
an unmixed specimen of each group was centrifuged/measured after 3,
6, 24 and 72 h. Results: TIMP-1 increased after freeze/ thaw (p
< 0.001). Mixing blood specimens more than once caused increased
TIMP-1 (p < 0.001). TIMP-1 increased within 3 h of storage (p
< 0.001). The increase was lower in specimens covered and
refrigerated (p < 0.001). Conclusion: TIMP-1 is unstable and has
to be evaluated carefully. Blood should be centrifuged directly
after venipuncture. For routine application, specimen handling must
be standardized and carefully followed. Research should be done on
specimens handled identically. Copyright (C) 2008 S. Karger AG,
Basel.

Weitere Episoden

Report of the Working Group `Overall Blood Supply Strategy with Regard to Variant Creutzfeldt-Jakob Disease (vCJD)'

Report of the Working Group `Overall Blood Supply Strategy with Regard to Variant Creutzfeldt-Jakob Disease (vCJD)'

vor 17 Jahren
Intranasal insulin to improve developmental delay in children with 22q13 deletion syndrome: an exploratory clinical trial

Intranasal insulin to improve developmental delay in children with 22q13 deletion syndrome: an exploratory clinical trial

vor 17 Jahren
Guidelines for initiation of anti-tumour necrosis factor therapy in rheumatoid arthritis: similarities and differences across Europe.

Guidelines for initiation of anti-tumour necrosis factor therapy in rheumatoid arthritis: similarities and differences across Europe.

vor 17 Jahren
JNK inhibition sensitises hepatocellular carcinoma cells but not normal hepatocytes to the TNF-related apoptosis-inducing ligand.

JNK inhibition sensitises hepatocellular carcinoma cells but not normal hepatocytes to the TNF-related apoptosis-inducing ligand.

vor 17 Jahren
Influence of peptidylarginine deiminase type 4 genotype and shared epitope on clinical characteristics and autoantibody profile of rheumatoid arthritis.

Influence of peptidylarginine deiminase type 4 genotype and shared epitope on clinical characteristics and autoantibody profile of rheumatoid arthritis.

vor 17 Jahren
Alle Episoden anzeigen
In Podcasts werben
Schalte jetzt Werbung in Podcasts.
Episode melden

„Dependence of TIMP-1 plasma levels on preanalytical specimen handling“

Worum geht es? Danach fragen wir noch nach dem Grund.

Abonnenten

Teilen

Mein Archiv

Deine Privatkopie der Folgen, die du nicht verlieren willst.

Podcast-Folgen verschwinden. Feeds werden auf die letzten Episoden gekürzt, Hoster räumen alte Dateien ab, Formate wechseln den Anbieter und lassen ihr Archiv zurück. Mit „Mein Archiv“ sichert podcast.de die Folgen deiner Podcasts für dich — angefangen bei den ältesten, denn die sind zuerst weg.

  • Deine gesicherten Folgen bleiben hörbar, auch wenn das Original offline geht.
  • Auch Folgen, die im heutigen Feed gar nicht mehr stehen — podcast.de kennt sie noch.
  • Herunterladen bleibt möglich, solange die Folge beim Podcaster liegt. Der zählt seine Abrufe wie bisher.
Startet bald

Sei beim Start von Mein Archiv dabei

Mein Archiv ist fast fertig. Trag dich ein, dann bekommst du eine E-Mail, sobald es losgeht – und bist von Anfang an dabei. Wir schreiben dir nur zum Start, keine Werbung, keine Weitergabe deiner Daten.

Du bekommst zuerst eine Bestätigungsmail. Abmelden geht jederzeit. Datenschutz