Der Einfluss des α2-Adrenozeptor Agonisten Dexmedetomidin auf die Expression Apoptose-assoziierter Proteine nach inkompletter zerebraler Hemisphärenischämie bei der Ratte im protrahierten zeitlichen Verlauf

Long-term effects of Dexmedetomidin on the expression of
apoptosis-regulating proteins after incomplete cerebral ischemia in
rats This study investigates the effect of the α2-adrenozeptor
agonist Dexmedetomidine on the expression of the
apoptosis-regulating proteins Bax, p53, Bcl-2 and Mdm-2 following
incomplete cerebral ischemia in rats within a period of 28 days
from the insult. 72 fasted male Sprague-Dawley rats (400 g) were
randomly assigned to one of the following groups: Group 1: (n = 32,
controls): fentanyl and N2O/O2 (FiO2 = 0.33) Group 2: (n = 32,
dexmedetomidine) : fentanyl and N2O/O2 (FiO2 = 0.33),
administration of dexmedetomidine intraperitoneal 30 min before
ischemia, the animals of these groups were randomly assigned in
groups (n = 8) with a postischemic observation period of 1, 3, 7 or
28 days. Group 3: (n = 8, naive): without treatment, show reference
value The apoptosis-regulating proteins Bax, p53, Bcl-2 and Mdm-2
in the hippocampal regions were analysed qualitatively and
quantitatively by using the Immunfluorescence-technique and the
Western-Blot-technique. The concentration of the pro-apoptotic
protein Bax is decreased in the hippocampus for at least 28 days
after cerebral ischemia. The anti-apototic Bcl-2 protein was
increased during the first three days after cerebral ischemia in
group 2 compared to group 1. The Mdm-2 protein of group 2 was
significantly increased during the whole period of investigation in
the Western-Blot-analysis.