Evaluierung von Liquorpunktion und PCR zur klinischen Diagnose der Enzephalitozoonose beim Kaninchen
Beschreibung
vor 19 Jahren
The purpose of the present study was the evaluation of the clinical
utility of CSF analysis and PCR for the diagnosis of the
neurological type of encephalitozoonosis in rabbits. A total of 46
rabbits with neurological symptoms due to encephalitozoonose were
included in the study. CSF was examined in 21 rabbits with
encephalitozoonosis and 20 healthy laboratory rabbits. In all
rabbits collection of CSF was easy to perform. Few rabbits with
vestibular disease showed short-term deterioration of neurological
symptoms which were completely reversed after eight hours. CSF
analysis of laboratory rabbits revealed WBC counts from 0 to 4
cells/µl (median 1.5 cells/µl). The protein level ranged from 0.13
to 0.31 g/l (median 0.24 g/l). In contrast to this, pet rabbits
with the neurological type of encephalitozoonosis showed distinct
monocytic-lymphocytic pleocytosis with WBC counts from 5 to 78
cells/µl (median 15.3 cells/µl) and increased protein levels from
0.31 to 1.54 g/l (median 0.69 g/l). Compared to healthy rabbits,
distinction for both parameters was highly significant (p <
0.001). It was concluded that mononuclear pleocytosis and elevated
protein is a regular feature of rabbit encephalitozoonosis. PCR for
detection of E. cuniculi DNA as used in this study was based on an
established protocol (Katzwinkel-Wladarsch et al. 1996).
PCR-testing of CSF revealed positive results only in two of 19
samples (10.5 %), while for urine 15 of 38 samples (39.5 %) tested
positive for E. cuniculi-DNA. With microscopy after trichrome
stain, however, E. cuniculi spores were detected only in four out
of 38 urine samples (10.5 %). All results of trichrome stain
correlated with those of PCR. In conclusion, PCR of CSF is no help
for diagnosis of encephalitozoonosis in rabbits. PCR for detection
of E. cuniculi in urine on the other hand, was found to be far more
sensitive than trichrome stain. To determine sensitivity and
specificity of this PCR in urine, future studies should include
larger numbers of rabbits and those with clinical renal disease.
utility of CSF analysis and PCR for the diagnosis of the
neurological type of encephalitozoonosis in rabbits. A total of 46
rabbits with neurological symptoms due to encephalitozoonose were
included in the study. CSF was examined in 21 rabbits with
encephalitozoonosis and 20 healthy laboratory rabbits. In all
rabbits collection of CSF was easy to perform. Few rabbits with
vestibular disease showed short-term deterioration of neurological
symptoms which were completely reversed after eight hours. CSF
analysis of laboratory rabbits revealed WBC counts from 0 to 4
cells/µl (median 1.5 cells/µl). The protein level ranged from 0.13
to 0.31 g/l (median 0.24 g/l). In contrast to this, pet rabbits
with the neurological type of encephalitozoonosis showed distinct
monocytic-lymphocytic pleocytosis with WBC counts from 5 to 78
cells/µl (median 15.3 cells/µl) and increased protein levels from
0.31 to 1.54 g/l (median 0.69 g/l). Compared to healthy rabbits,
distinction for both parameters was highly significant (p <
0.001). It was concluded that mononuclear pleocytosis and elevated
protein is a regular feature of rabbit encephalitozoonosis. PCR for
detection of E. cuniculi DNA as used in this study was based on an
established protocol (Katzwinkel-Wladarsch et al. 1996).
PCR-testing of CSF revealed positive results only in two of 19
samples (10.5 %), while for urine 15 of 38 samples (39.5 %) tested
positive for E. cuniculi-DNA. With microscopy after trichrome
stain, however, E. cuniculi spores were detected only in four out
of 38 urine samples (10.5 %). All results of trichrome stain
correlated with those of PCR. In conclusion, PCR of CSF is no help
for diagnosis of encephalitozoonosis in rabbits. PCR for detection
of E. cuniculi in urine on the other hand, was found to be far more
sensitive than trichrome stain. To determine sensitivity and
specificity of this PCR in urine, future studies should include
larger numbers of rabbits and those with clinical renal disease.
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