Untersuchung des Stellenwertes der Messung spezifischer Antikörper im Liquor cerebrospinalis für die Diagnose der ZNS-Manifestation der Felinen Infektiösen Peritonitis
Beschreibung
vor 19 Jahren
Investigation of anti-coronavirus-antibodies in the cerebrospinal
fluid for the diagnosis of CNS manifestation of feline infectious
peritonitis The purpose of the present study was to evaluate the
occurence of anti-coronavirus antibodies in the cerebrospinal fluid
(CSF) of cats and to assess their diagnostic utility concerning the
central nervous system (CNS) manifestation of feline infectious
peritonitis (FIP). Anti-coronavirus immunoglobulin G (IgG) was
measured in CSF and serum of 67 cats by indirect immunofluorescence
assay. Additionally, cell counts, Pandy’s test and total protein
were performed in the CSF. Diagnosis of all cats was confirmed by
gross and histological examination including the brain. Four
different groups were examined: FIP with CNS manifestation (n =
10), FIP without CNS manifestation (n = 13), other CNS disorders
than FIP (n = 29) and diseases not associated with FIP and not
involving the CNS (n = 15). Anti-coronavirus IgG was detected in
the CSF of 18% of all cats (12/67): 6 cats with FIP involving the
CNS, 4 cats with FIP not involving the CNS and 2 cats with brain
tumours. CSF antibodies were only found in seropositive cats. In
addition, only cats with serum titers equal or higher than 1:4096
showed CSF titers. The correlation between serum antibodies and CSF
antibodies was statistically highly significant (r=0,652; p=0,000).
No statistically significant correlation was found between the
erythrocyte count and CSF titers. However, a certain tendency for
high CSF titers with increasing numbers of erythrocytes was
observed. For all cats of the study (n = 67), testing for
anti-coronavirus IgG in the CSF showed a sensitivity of 60% and a
specificity of 90%. CSF antibodies had a positive predictive value
of 50% for the CNS manifestation of FIP. The negative predictive
value was 93%. Calculating only the values of cats with CNS
alterations (n = 39), testing for anti-coronavirus IgG in the CSF
had a sensitivity of 60% and a specificity of 93%. The positive and
negative predictive value was 75% and 87%, respectively. The
present study revealed that the determination of
anti-coronavirus-titers in the CSF alone does not confirm CNS
manifestation of FIP. The statistically highly significant
correlation between CSF and serum antibodies indicates a disrupted
blood brain barrier or blood contamination. Future studies on CSF
anti-coronavirus-antibodies should address the evaluation of the
status of the blood brain barrier and the measurement of the
intrathecally produced antibody portion.
fluid for the diagnosis of CNS manifestation of feline infectious
peritonitis The purpose of the present study was to evaluate the
occurence of anti-coronavirus antibodies in the cerebrospinal fluid
(CSF) of cats and to assess their diagnostic utility concerning the
central nervous system (CNS) manifestation of feline infectious
peritonitis (FIP). Anti-coronavirus immunoglobulin G (IgG) was
measured in CSF and serum of 67 cats by indirect immunofluorescence
assay. Additionally, cell counts, Pandy’s test and total protein
were performed in the CSF. Diagnosis of all cats was confirmed by
gross and histological examination including the brain. Four
different groups were examined: FIP with CNS manifestation (n =
10), FIP without CNS manifestation (n = 13), other CNS disorders
than FIP (n = 29) and diseases not associated with FIP and not
involving the CNS (n = 15). Anti-coronavirus IgG was detected in
the CSF of 18% of all cats (12/67): 6 cats with FIP involving the
CNS, 4 cats with FIP not involving the CNS and 2 cats with brain
tumours. CSF antibodies were only found in seropositive cats. In
addition, only cats with serum titers equal or higher than 1:4096
showed CSF titers. The correlation between serum antibodies and CSF
antibodies was statistically highly significant (r=0,652; p=0,000).
No statistically significant correlation was found between the
erythrocyte count and CSF titers. However, a certain tendency for
high CSF titers with increasing numbers of erythrocytes was
observed. For all cats of the study (n = 67), testing for
anti-coronavirus IgG in the CSF showed a sensitivity of 60% and a
specificity of 90%. CSF antibodies had a positive predictive value
of 50% for the CNS manifestation of FIP. The negative predictive
value was 93%. Calculating only the values of cats with CNS
alterations (n = 39), testing for anti-coronavirus IgG in the CSF
had a sensitivity of 60% and a specificity of 93%. The positive and
negative predictive value was 75% and 87%, respectively. The
present study revealed that the determination of
anti-coronavirus-titers in the CSF alone does not confirm CNS
manifestation of FIP. The statistically highly significant
correlation between CSF and serum antibodies indicates a disrupted
blood brain barrier or blood contamination. Future studies on CSF
anti-coronavirus-antibodies should address the evaluation of the
status of the blood brain barrier and the measurement of the
intrathecally produced antibody portion.
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