podcast.de
Anmeldung
Anmeldung Registrierung
Auto Hell Dunkel
Erweiterte Suche
  1. Startseite
  2. Podcasts
  3. Medizin - Open Access LMU - Teil 14/22 Podcast
  4. Measurement of late-night salivary cortisol with an automated immunoassay system
Background: Measurement of late-night salivary cortisol
concentrations is increasingly used as a screening test in
suspected Cushing's syndrome. Cortisol concentrations are typically
extremely low in late-night samples and discordant assay-specific
reference ranges have been reported. Therefore, the aim of our
study was to assess the analytical performance of the first
automated cortisol immunoassay specified for salivary measurements
and to establish late-night sampling reference-range data for this
test. Methods: Salivary cortisol was measured using the Roche Cobas
Cortisol assay (Roche Diagnostics). Five salivary pools in
different concentration ranges were used to assess the inter-assay
imprecision of this test in a two-centre evaluation protocol
including two reagent lots. Linearity was tested by serial
dilution. Salivary samples were obtained at 23:00 h from 100
apparently healthy volunteers using a commercially available
salivary sampling device (Salivette, Sarstedt). A subset of 20
samples was used for method comparison with isotope dilution liquid
chromatography-tandem mass spectrometry. Results: Inter-assay
coefficients of variation (n=20) between 11.6% and 40.4% were found
for mean cortisol concentrations between 12.9 and 2.6 nmol/L, with
an estimated functional sensitivity of approximately 5.0 nmol/L.
The test also gave linear results in the lowest concentration range
between 1.0 and 8.3 nmol/L. Mean late-night salivary cortisol of
5.0 nmol/L was found for healthy individuals; the absolute range
was 1.4-16.7 nmol/L, and the 95th percentile was 8.9 nmol/L.
Substantially lower concentrations were found with isotope dilution
LC-MS/MS compared to immunoassay results (mean concentrations 1.8
and 4.4 nmol/L, respectively). Conclusions: The automated assay
investigated was found to offer acceptable analytical performance
in the very low concentration range required for late-night
salivary cortisol, despite a very short turnaround time. Using this
assay, late-night salivary cortisol concentrations below 8.9 nmol/L
are typically found in healthy volunteers.

Weitere Episoden

External quality assessment of urinary methylmalonic acid quantification - results of a pilot study

External quality assessment of urinary methylmalonic acid quantification - results of a pilot study

vor 19 Jahren
Use of novel serum markers in clinical follow-up of Sertoli-Leydig cell turnours

Use of novel serum markers in clinical follow-up of Sertoli-Leydig cell turnours

vor 19 Jahren
Free serum cortisol: quantification applying equilibrium dialysis or ultrafiltration and an automated immunoassay system

Free serum cortisol: quantification applying equilibrium dialysis or ultrafiltration and an automated immunoassay system

vor 19 Jahren
Die Biegestabprothese: ein experimenteller Ansatz zur metaphysären Hüftendoprothetik

Die Biegestabprothese: ein experimenteller Ansatz zur metaphysären Hüftendoprothetik

vor 19 Jahren
ASCO 2007: What remains important for breast cancer systemic therapy in the routine setting?

ASCO 2007: What remains important for breast cancer systemic therapy in the routine setting?

vor 19 Jahren
Alle Episoden anzeigen
In Podcasts werben
Schalte jetzt Werbung in Podcasts.
Episode melden

„Measurement of late-night salivary cortisol with an automated immunoassay system“

Worum geht es? Danach fragen wir noch nach dem Grund.

Abonnenten

Teilen

Mein Archiv

Deine Privatkopie der Folgen, die du nicht verlieren willst.

Podcast-Folgen verschwinden. Feeds werden auf die letzten Episoden gekürzt, Hoster räumen alte Dateien ab, Formate wechseln den Anbieter und lassen ihr Archiv zurück. Mit „Mein Archiv“ sichert podcast.de die Folgen deiner Podcasts für dich — angefangen bei den ältesten, denn die sind zuerst weg.

  • Deine gesicherten Folgen bleiben hörbar, auch wenn das Original offline geht.
  • Auch Folgen, die im heutigen Feed gar nicht mehr stehen — podcast.de kennt sie noch.
  • Herunterladen bleibt möglich, solange die Folge beim Podcaster liegt. Der zählt seine Abrufe wie bisher.
Startet bald

Sei beim Start von Mein Archiv dabei

Mein Archiv ist fast fertig. Trag dich ein, dann bekommst du eine E-Mail, sobald es losgeht – und bist von Anfang an dabei. Wir schreiben dir nur zum Start, keine Werbung, keine Weitergabe deiner Daten.

Du bekommst zuerst eine Bestätigungsmail. Abmelden geht jederzeit. Datenschutz