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Medizin - Open Access LMU - Teil 09/22 Podcast
Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries

Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries

Medizin - Open Access LMU - Teil 09/22 vor 34 Jahren

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We established chromosomal homologies between all chromosomes of
the human karyotype and that of an old world monkey (Macaca
fuscata) by chromosomal in situ suppression (CISS) hybridization
with human chromosome specific DNA libraries. Except for the human
chromosome 2 library and limited cross-hybridization of X and Y
chromosome libraries all human DNA libraries hybridized to single
GTG-banded macaque chromosomes. Only three macaque chromosomes (2,
7, 13) were each hybridized by two separate human libraries (7 and
21, 14 and 15, 20 and 22 respectively). Thus, an unequivocally high
degree of synteny between human and macaque chromosomes has been
maintained for more than 20 million years. As previously suggested,
both Papionini (macaques, baboons, mandrills and cercocebus
monkeys, all of which have nearly identical karyotypes) and humans
are chromosomally conservative. The results suggest, that CISS
hybridization can be expected to become an indispensable tool in
comparative chromosome and gene mapping and will help clarify
chromosomal phylogenies with speed and accuracy.