Translocation (8;21) in acute nonlymphocytic leukemia delineated by chromosomal in situ suppression hybridization

In situ suppression hybridization with recombinant bacteriophage
DNA libraries for chromosomes 8 and 21 was performed in two cases
of acute nonlymphocytic leukemia, type FAB M2. In both cases,
cytogenetic analysis by conventional G-banding revealed
t(8;21)(q22;q22). In situ suppression hybridization was able to
prove the reciprocal nature of the translocation in both cases by
identifying the terminal end of chromosome 21 translocated to the
derivative chromosome 8q−.