podcast.de
Anmeldung
Anmeldung Registrierung
Auto Hell Dunkel
Erweiterte Suche
  1. Startseite
  2. Podcasts
  3. Medizin - Open Access LMU - Teil 17/22 Podcast
  4. Directing astroglia from the cerebral cortex into subtype specific functional neurons
Astroglia from the postnatal cerebral cortex can be reprogrammed in
vitro to generate neurons following forced expression of neurogenic
transcription factors, thus opening new avenues towards a potential
use of endogenous astroglia for brain repair. However, in previous
attempts astroglia-derived neurons failed to establish functional
synapses, a severe limitation towards functional neurogenesis. It
remained therefore also unknown whether neurons derived from
reprogrammed astroglia could be directed towards distinct neuronal
subtype identities by selective expression of distinct neurogenic
fate determinants. Here we show that strong and persistent
expression of neurogenic fate determinants driven by
silencing-resistant retroviral vectors instructs astroglia from the
postnatal cortex in vitro to mature into fully functional,
synapse-forming neurons. Importantly, the neurotransmitter fate
choice of astroglia-derived neurons can be controlled by selective
expression of distinct neurogenic transcription factors: forced
expression of the dorsal telencephalic fate determinant
neurogenin-2 (Neurog2) directs cortical astroglia to generate
synapse-forming glutamatergic neurons; in contrast, the ventral
telencephalic fate determinant Dlx2 induces a GABAergic identity,
although the overall efficiency of Dlx2-mediated neuronal
reprogramming is much lower compared to Neurog2, suggesting that
cortical astroglia possess a higher competence to respond to the
dorsal telencephalic fate determinant. Interestingly, however,
reprogramming of astroglia towards the generation of GABAergic
neurons was greatly facilitated when the astroglial cells were
first expanded as neurosphere cells prior to transduction with
Dlx2. Importantly, this approach of expansion under neurosphere
conditions and subsequent reprogramming with distinct neurogenic
transcription factors can also be extended to reactive astroglia
isolated from the adult injured cerebral cortex, allowing for the
selective generation of glutamatergic or GABAergic neurons. These
data provide evidence that cortical astroglia can undergo a
conversion across cell lineages by forced expression of a single
neurogenic transcription factor, stably generating fully
differentiated neurons. Moreover, neuronal reprogramming of
astroglia is not restricted to postnatal stages but can also be
achieved from terminally differentiated astroglia of the adult
cerebral cortex following injury-induced reactivation.

Weitere Episoden

Analysis of IL2/IL21 Gene Variants in Cholestatic Liver Diseases Reveals an Association with Primary Sclerosing Cholangitis

Analysis of IL2/IL21 Gene Variants in Cholestatic Liver Diseases Reveals an Association with Primary Sclerosing Cholangitis

vor 15 Jahren
Biliary Bicarbonate Secretion Constitutes a Protective Mechanism against Bile Acid-Induced Injury in Man

Biliary Bicarbonate Secretion Constitutes a Protective Mechanism against Bile Acid-Induced Injury in Man

vor 15 Jahren
Growth Pattern of Untreated Boys with Simple Virilizing Congenital Adrenal Hyperplasia Indicates Relative Androgen Insensitivity during the First Six Months of Life

Growth Pattern of Untreated Boys with Simple Virilizing Congenital Adrenal Hyperplasia Indicates Relative Androgen Insensitivity during the First Six Months of Life

vor 15 Jahren
Growth Patterns in the First Three Years of Life in Children with Classical Congenital Adrenal Hyperplasia Diagnosed by Newborn Screening and Treated with Low Doses of Hydrocortisone

Growth Patterns in the First Three Years of Life in Children with Classical Congenital Adrenal Hyperplasia Diagnosed by Newborn Screening and Treated with Low Doses of Hydrocortisone

vor 15 Jahren
Missing Heritability in the Tails of Quantitative Traits? A Simulation Study on the Impact of Slightly Altered True Genetic Models

Missing Heritability in the Tails of Quantitative Traits? A Simulation Study on the Impact of Slightly Altered True Genetic Models

vor 15 Jahren
Alle Episoden anzeigen
In Podcasts werben
Schalte jetzt Werbung in Podcasts.
Episode melden

„Directing astroglia from the cerebral cortex into subtype specific functional neurons“

Worum geht es? Danach fragen wir noch nach dem Grund.

Abonnenten

Er
Erasmus2

Teilen

Mein Archiv

Deine Privatkopie der Folgen, die du nicht verlieren willst.

Podcast-Folgen verschwinden. Feeds werden auf die letzten Episoden gekürzt, Hoster räumen alte Dateien ab, Formate wechseln den Anbieter und lassen ihr Archiv zurück. Mit „Mein Archiv“ sichert podcast.de die Folgen deiner Podcasts für dich — angefangen bei den ältesten, denn die sind zuerst weg.

  • Deine gesicherten Folgen bleiben hörbar, auch wenn das Original offline geht.
  • Auch Folgen, die im heutigen Feed gar nicht mehr stehen — podcast.de kennt sie noch.
  • Herunterladen bleibt möglich, solange die Folge beim Podcaster liegt. Der zählt seine Abrufe wie bisher.
Startet bald

Sei beim Start von Mein Archiv dabei

Mein Archiv ist fast fertig. Trag dich ein, dann bekommst du eine E-Mail, sobald es losgeht – und bist von Anfang an dabei. Wir schreiben dir nur zum Start, keine Werbung, keine Weitergabe deiner Daten.

Du bekommst zuerst eine Bestätigungsmail. Abmelden geht jederzeit. Datenschutz