podcast.de
Anmeldung
Anmeldung Registrierung
Auto Hell Dunkel
Erweiterte Suche
  1. Startseite
  2. Podcasts
  3. Medizin - Open Access LMU - Teil 17/22 Podcast
  4. Assessment of Surfactant Protein A (SP-A) dependent agglutination
Background: Monomers of the collectin surfactant associated
protein-A (SP-A) are arranged in trimers and higher oligomers. The
state of oligomerization differs between individuals and likely
affects SP-A's functional properties. SP-A can form aggregates
together with other SP-A molecules. Here we report and assess a
test system for the aggregate forming properties of SP-A in serum
and broncho-alveolar lavage samples. Methods: Anti-SP-A antibodies
fixed to latex beads bound SP-A at its N-terminal end and allowed
the interaction with other SP-A molecules in a given sample by
their C-terminal carbohydrate recognition domain (CRD) to
agglutinate the beads to aggregates, which were quantified by light
microscopy. Results: SP-A aggregation was dependent on its
concentration, the presence of calcium, and was dose-dependently
inhibited by mannose. Unaffected by the presence of SP-D no
aggregation was observed in absence of SP-A. The more complex the
oligomeric structure of SP-A present in a particular sample, the
better was its capability to induce aggregation at a given total
concentration of SP-A. SP-A in serum agglutinated independently of
the pulmonary disease; in contrast SP-A in lung lavage fluid was
clearly inferior in patients with chronic bronchitis and
particularly with cystic fibrosis compared to controls.
Conclusions: The functional status of SP-A with respect to its
aggregating properties in serum and lavage samples can be easily
assessed. SP-A in lung lavage fluid in patients with severe
neutrophilic bronchitis was inferior.

Weitere Episoden

Analysis of IL2/IL21 Gene Variants in Cholestatic Liver Diseases Reveals an Association with Primary Sclerosing Cholangitis

Analysis of IL2/IL21 Gene Variants in Cholestatic Liver Diseases Reveals an Association with Primary Sclerosing Cholangitis

vor 15 Jahren
Biliary Bicarbonate Secretion Constitutes a Protective Mechanism against Bile Acid-Induced Injury in Man

Biliary Bicarbonate Secretion Constitutes a Protective Mechanism against Bile Acid-Induced Injury in Man

vor 15 Jahren
Growth Pattern of Untreated Boys with Simple Virilizing Congenital Adrenal Hyperplasia Indicates Relative Androgen Insensitivity during the First Six Months of Life

Growth Pattern of Untreated Boys with Simple Virilizing Congenital Adrenal Hyperplasia Indicates Relative Androgen Insensitivity during the First Six Months of Life

vor 15 Jahren
Growth Patterns in the First Three Years of Life in Children with Classical Congenital Adrenal Hyperplasia Diagnosed by Newborn Screening and Treated with Low Doses of Hydrocortisone

Growth Patterns in the First Three Years of Life in Children with Classical Congenital Adrenal Hyperplasia Diagnosed by Newborn Screening and Treated with Low Doses of Hydrocortisone

vor 15 Jahren
Missing Heritability in the Tails of Quantitative Traits? A Simulation Study on the Impact of Slightly Altered True Genetic Models

Missing Heritability in the Tails of Quantitative Traits? A Simulation Study on the Impact of Slightly Altered True Genetic Models

vor 15 Jahren
Alle Episoden anzeigen
In Podcasts werben
Schalte jetzt Werbung in Podcasts.
Episode melden

„Assessment of Surfactant Protein A (SP-A) dependent agglutination“

Worum geht es? Danach fragen wir noch nach dem Grund.

Abonnenten

Er
Erasmus2

Teilen

Mein Archiv

Deine Privatkopie der Folgen, die du nicht verlieren willst.

Podcast-Folgen verschwinden. Feeds werden auf die letzten Episoden gekürzt, Hoster räumen alte Dateien ab, Formate wechseln den Anbieter und lassen ihr Archiv zurück. Mit „Mein Archiv“ sichert podcast.de die Folgen deiner Podcasts für dich — angefangen bei den ältesten, denn die sind zuerst weg.

  • Deine gesicherten Folgen bleiben hörbar, auch wenn das Original offline geht.
  • Auch Folgen, die im heutigen Feed gar nicht mehr stehen — podcast.de kennt sie noch.
  • Herunterladen bleibt möglich, solange die Folge beim Podcaster liegt. Der zählt seine Abrufe wie bisher.
Startet bald

Sei beim Start von Mein Archiv dabei

Mein Archiv ist fast fertig. Trag dich ein, dann bekommst du eine E-Mail, sobald es losgeht – und bist von Anfang an dabei. Wir schreiben dir nur zum Start, keine Werbung, keine Weitergabe deiner Daten.

Du bekommst zuerst eine Bestätigungsmail. Abmelden geht jederzeit. Datenschutz