podcast.de
Anmeldung
Anmeldung Registrierung
Auto Hell Dunkel
Erweiterte Suche
  1. Startseite
  2. Podcasts
  3. Medizin - Open Access LMU - Teil 17/22 Podcast
  4. Cold storage and cryopreservation of tick cell lines
Background: Tick cell lines are now available from fifteen ixodid
and argasid species of medical and veterinary importance. However,
some tick cell lines can be difficult to cryopreserve, and improved
protocols for short- and long-term low temperature storage will
greatly enhance their use as tools in tick and tick-borne pathogen
research. In the present study, different protocols were evaluated
for cold storage and cryopreservation of tick cell lines derived
from Rhipicephalus (Boophilus) decoloratus, Rhipicephalus
(Boophilus) microplus, Ixodes ricinus and Ixodes scapularis. For
short-term cold storage, cells were kept under refrigeration at 6 C
for 15, 30 and 45 days. For cryopreservation in liquid nitrogen,
use of a sucrose-phosphate-glutamate freezing buffer (SPG) as
cryoprotectant was compared with dimethylsulfoxide (DMSO)
supplemented with sucrose. Cell viability was determined by the
trypan blue exclusion test and cell morphology was evaluated in
Giemsa-stained cytocentrifuge smears. Results: Cold storage at 6
degrees C for up to 30 days was successful in preserving R. (B.)
microplus, R. (B.) decoloratus, I. ricinus and I. scapularis cell
lines; lines from the latter three species could be easily
re-cultivated after 45 days under refrigeration. While cell lines
from all four tick species cryopreserved with 6% DMSO were
successfully resuscitated, the R. (B.) decoloratus cells did not
survive freezing in SPG and of the other three species, only the R.
(B.) microplus cells resumed growth during the observation period.
Conclusions: This constitutes the first report on successful
short-term refrigeration of cells derived from R. (B.) decoloratus,
R. (B.) microplus, and I. ricinus, and use of SPG as an alternative
to DMSO for cryopreservation, thus making an important contribution
to more reliable and convenient tick cell culture maintenance.

Weitere Episoden

Analysis of IL2/IL21 Gene Variants in Cholestatic Liver Diseases Reveals an Association with Primary Sclerosing Cholangitis

Analysis of IL2/IL21 Gene Variants in Cholestatic Liver Diseases Reveals an Association with Primary Sclerosing Cholangitis

vor 15 Jahren
Biliary Bicarbonate Secretion Constitutes a Protective Mechanism against Bile Acid-Induced Injury in Man

Biliary Bicarbonate Secretion Constitutes a Protective Mechanism against Bile Acid-Induced Injury in Man

vor 15 Jahren
Growth Pattern of Untreated Boys with Simple Virilizing Congenital Adrenal Hyperplasia Indicates Relative Androgen Insensitivity during the First Six Months of Life

Growth Pattern of Untreated Boys with Simple Virilizing Congenital Adrenal Hyperplasia Indicates Relative Androgen Insensitivity during the First Six Months of Life

vor 15 Jahren
Growth Patterns in the First Three Years of Life in Children with Classical Congenital Adrenal Hyperplasia Diagnosed by Newborn Screening and Treated with Low Doses of Hydrocortisone

Growth Patterns in the First Three Years of Life in Children with Classical Congenital Adrenal Hyperplasia Diagnosed by Newborn Screening and Treated with Low Doses of Hydrocortisone

vor 15 Jahren
Missing Heritability in the Tails of Quantitative Traits? A Simulation Study on the Impact of Slightly Altered True Genetic Models

Missing Heritability in the Tails of Quantitative Traits? A Simulation Study on the Impact of Slightly Altered True Genetic Models

vor 15 Jahren
Alle Episoden anzeigen
In Podcasts werben
Schalte jetzt Werbung in Podcasts.
Episode melden

„Cold storage and cryopreservation of tick cell lines“

Worum geht es? Danach fragen wir noch nach dem Grund.

Abonnenten

Er
Erasmus2

Teilen

Mein Archiv

Deine Privatkopie der Folgen, die du nicht verlieren willst.

Podcast-Folgen verschwinden. Feeds werden auf die letzten Episoden gekürzt, Hoster räumen alte Dateien ab, Formate wechseln den Anbieter und lassen ihr Archiv zurück. Mit „Mein Archiv“ sichert podcast.de die Folgen deiner Podcasts für dich — angefangen bei den ältesten, denn die sind zuerst weg.

  • Deine gesicherten Folgen bleiben hörbar, auch wenn das Original offline geht.
  • Auch Folgen, die im heutigen Feed gar nicht mehr stehen — podcast.de kennt sie noch.
  • Herunterladen bleibt möglich, solange die Folge beim Podcaster liegt. Der zählt seine Abrufe wie bisher.
Startet bald

Sei beim Start von Mein Archiv dabei

Mein Archiv ist fast fertig. Trag dich ein, dann bekommst du eine E-Mail, sobald es losgeht – und bist von Anfang an dabei. Wir schreiben dir nur zum Start, keine Werbung, keine Weitergabe deiner Daten.

Du bekommst zuerst eine Bestätigungsmail. Abmelden geht jederzeit. Datenschutz