podcast.de
Anmeldung
Anmeldung Registrierung
Auto Hell Dunkel
Erweiterte Suche
  1. Startseite
  2. Podcasts
  3. Medizin - Open Access LMU - Teil 17/22 Podcast
  4. Colitis induced in mice with dextran sulfate sodium (DSS) is mediated by the NLRP3 inflammasome
Background The proinflammatory cytokines interleukin 1 beta (IL-1
beta) and IL-18 are central players in the pathogenesis of
inflammatory bowel disease (IBD). In response to a variety of
microbial components and crystalline substances, both cytokines are
processed via the caspase-1-activating multiprotein complex, the
NLRP3 inflammasome. Here, the role of the NLRP3 inflammasome in
experimental colitis induced by dextran sodium sulfate (DSS) was
examined. Methods IL-1b production in response to DSS was studied
in macrophages of wild-type, caspase-1(-/-), NLRP3(-/-), ASC(-/-),
cathepsin B(-/-) or cathepsin L(-/-) mice. Colitis was induced in
C57BL/6 and NLRP3(-/-) mice by oral DSS administration. A clinical
disease activity score was evaluated daily. Histological colitis
severity and expression of cytokines were determined in colonic
tissue. Results Macrophages incubated with DSS in vitro secreted
high levels of IL-1b in a caspase-1-dependent manner. IL-1b
secretion was abrogated in macrophages lacking NLRP3, ASC or
caspase-1, indicating that DSS activates caspase-1 via the NLRP3
inflammasome. Moreover, IL-1b secretion was dependent on
phagocytosis, lysosomal maturation, cathepsin B and L, and reactive
oxygen species (ROS). After oral administration of DSS, NLRP3(-/-)
mice developed a less severe colitis than wild-type mice and
produced lower levels of proinflammatory cytokines in colonic
tissue. Pharmacological inhibition of caspase-1 with pralnacasan
achieved a level of mucosal protection comparable with NLRP3
deficiency. Conclusions The NLRP3 inflammasome was identified as a
critical mechanism of intestinal inflammation in the DSS colitis
model. The NLRP3 inflammasome may serve as a potential target for
the development of novel therapeutics for patients with IBD.

Weitere Episoden

Analysis of IL2/IL21 Gene Variants in Cholestatic Liver Diseases Reveals an Association with Primary Sclerosing Cholangitis

Analysis of IL2/IL21 Gene Variants in Cholestatic Liver Diseases Reveals an Association with Primary Sclerosing Cholangitis

vor 15 Jahren
Biliary Bicarbonate Secretion Constitutes a Protective Mechanism against Bile Acid-Induced Injury in Man

Biliary Bicarbonate Secretion Constitutes a Protective Mechanism against Bile Acid-Induced Injury in Man

vor 15 Jahren
Growth Pattern of Untreated Boys with Simple Virilizing Congenital Adrenal Hyperplasia Indicates Relative Androgen Insensitivity during the First Six Months of Life

Growth Pattern of Untreated Boys with Simple Virilizing Congenital Adrenal Hyperplasia Indicates Relative Androgen Insensitivity during the First Six Months of Life

vor 15 Jahren
Growth Patterns in the First Three Years of Life in Children with Classical Congenital Adrenal Hyperplasia Diagnosed by Newborn Screening and Treated with Low Doses of Hydrocortisone

Growth Patterns in the First Three Years of Life in Children with Classical Congenital Adrenal Hyperplasia Diagnosed by Newborn Screening and Treated with Low Doses of Hydrocortisone

vor 15 Jahren
Missing Heritability in the Tails of Quantitative Traits? A Simulation Study on the Impact of Slightly Altered True Genetic Models

Missing Heritability in the Tails of Quantitative Traits? A Simulation Study on the Impact of Slightly Altered True Genetic Models

vor 15 Jahren
Alle Episoden anzeigen
In Podcasts werben
Schalte jetzt Werbung in Podcasts.
Episode melden

„Colitis induced in mice with dextran sulfate sodium (DSS) is mediated by the NLRP3 inflammasome“

Worum geht es? Danach fragen wir noch nach dem Grund.

Abonnenten

Er
Erasmus2

Teilen

Mein Archiv

Deine Privatkopie der Folgen, die du nicht verlieren willst.

Podcast-Folgen verschwinden. Feeds werden auf die letzten Episoden gekürzt, Hoster räumen alte Dateien ab, Formate wechseln den Anbieter und lassen ihr Archiv zurück. Mit „Mein Archiv“ sichert podcast.de die Folgen deiner Podcasts für dich — angefangen bei den ältesten, denn die sind zuerst weg.

  • Deine gesicherten Folgen bleiben hörbar, auch wenn das Original offline geht.
  • Auch Folgen, die im heutigen Feed gar nicht mehr stehen — podcast.de kennt sie noch.
  • Herunterladen bleibt möglich, solange die Folge beim Podcaster liegt. Der zählt seine Abrufe wie bisher.
Startet bald

Sei beim Start von Mein Archiv dabei

Mein Archiv ist fast fertig. Trag dich ein, dann bekommst du eine E-Mail, sobald es losgeht – und bist von Anfang an dabei. Wir schreiben dir nur zum Start, keine Werbung, keine Weitergabe deiner Daten.

Du bekommst zuerst eine Bestätigungsmail. Abmelden geht jederzeit. Datenschutz