Entwicklung eines ELISA zum Nachweis von Hepatitis E Antikörpern aus Serum und Fleischsaft des Schweins
Beschreibung
vor 13 Jahren
Reports on autochthonous Hepatitis E virus (HEV) infection in
humans with genotype 3 assume a zoonotic transmission from domestic
pigs. But for domestic pigs only limited information on the
seroprevalence of HEV in Germany is available. The aim of this
study was to develop an ELISA for the detection of anti-HEV IgG and
IgM in porcine serum and meat juice, and furthermore asses the
seroprevalence in domestic pigs from Bavaria, Germany. 516 serum
samples from pigs and 198 corresponding meat-juice samples from 41
different fattening units were collected in four Bavarian
slaughterhouses from august 2009 to february 2010. These samples
were tested for anti-HEV antibodies using the newly developed
recomWell HEV pig (Mikrogen GmbH, Neuried, Germany) with
recombinantly produced antigens of genotype 1 and genotype 3 of
HEV. The results were compared to a competitor ELISA (Axiom HEV Ab,
Axiom GmbH, Bürstadt, Germany). The data were verified by a HEV
line-immunoassay (recomLine HEV, Mikrogen), which also uses
antigens of genotype 1 and genotype 3 of HEV. Compared to the
line-immunoassay the Mikrogen / Axiom ELISA showed a sensitivity of
90.9% / 95.6% and a specificity of 94.0% / 80.8% respectively. The
correlation of optical densities (ODs) between serum and meat-juice
was 0.939. Taking all three antibody tests into account, we found
an overall anti-HEV IgG seroprevalence of 68.60%. 7.0% of the pigs
showed IgM antibodies against HEV. We found a significant
difference for two slaughterhouses concerning the seroprevalence
rates. One fattening unit was free of antibodies against HEV, nine
fattening units showed significant influence on the overall
seroprevalence rate. This is the first study showing a (very high)
anti-HEV reactivity rate in meat-juice of domestic pigs, which
corresponds to the seroprevalences measured consistently by three
different assays. A positivity rate of 7.0% for IgM has also never
been shown before. The used test-systems seem to be suitable for
antibody-testing in blood and meat-juice samples from domestic
pigs.
humans with genotype 3 assume a zoonotic transmission from domestic
pigs. But for domestic pigs only limited information on the
seroprevalence of HEV in Germany is available. The aim of this
study was to develop an ELISA for the detection of anti-HEV IgG and
IgM in porcine serum and meat juice, and furthermore asses the
seroprevalence in domestic pigs from Bavaria, Germany. 516 serum
samples from pigs and 198 corresponding meat-juice samples from 41
different fattening units were collected in four Bavarian
slaughterhouses from august 2009 to february 2010. These samples
were tested for anti-HEV antibodies using the newly developed
recomWell HEV pig (Mikrogen GmbH, Neuried, Germany) with
recombinantly produced antigens of genotype 1 and genotype 3 of
HEV. The results were compared to a competitor ELISA (Axiom HEV Ab,
Axiom GmbH, Bürstadt, Germany). The data were verified by a HEV
line-immunoassay (recomLine HEV, Mikrogen), which also uses
antigens of genotype 1 and genotype 3 of HEV. Compared to the
line-immunoassay the Mikrogen / Axiom ELISA showed a sensitivity of
90.9% / 95.6% and a specificity of 94.0% / 80.8% respectively. The
correlation of optical densities (ODs) between serum and meat-juice
was 0.939. Taking all three antibody tests into account, we found
an overall anti-HEV IgG seroprevalence of 68.60%. 7.0% of the pigs
showed IgM antibodies against HEV. We found a significant
difference for two slaughterhouses concerning the seroprevalence
rates. One fattening unit was free of antibodies against HEV, nine
fattening units showed significant influence on the overall
seroprevalence rate. This is the first study showing a (very high)
anti-HEV reactivity rate in meat-juice of domestic pigs, which
corresponds to the seroprevalences measured consistently by three
different assays. A positivity rate of 7.0% for IgM has also never
been shown before. The used test-systems seem to be suitable for
antibody-testing in blood and meat-juice samples from domestic
pigs.
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