Expansion von T-Zellen seltener Spezifität mittels B-Zellen

I concentrated on the expansion of T cells specific against the
oncogenic human papilloma virus 16 (HPV16), which are a very rare
population in the blood of healthy donors as well as patients with
HPV16-associated cancer. Instead of mature dendritic cells (DC) as
antigen presenting cells, which are a potent but limitated system,
I used activated B cells. I was able to expand cytotoxic active
HPV-specific T cells to therapeutically relevant cell numbers. I
analysed the T cell receptors of the E7-specific T cells well as
their avidity to get a first idea of clonality and avidity of
HPV-specific T cell pools.