Ein neues Tiermodell zur Analyse von Angiogenese und endokriner Funktion bei Transplantation von humanem Nebenschilddrüsengewebe auf die athyme Nacktmaus in vivo

It is unclear how angiogenesis and restoration of perfusion
determine graft function after free parathyroid
autotransplantation.We provide a new animal model allowing
simultaneous and repetitive in vivo assessment of angiogenesis and
endocrine function of parathyroid transplants. Methods:Fresh human
parathyroid tissue from patients with secondary hyperparathyroidism
was grafted into dorsal skinfold chamber preparations of athymic
nude mi(CD1-nu BR; n=8). Equivalent pieces of the same human donor
specimens were heat-inactivated and served as control grafts
(n=7).Results:In all animals receiving parathyroid transplants
human parathyroid hormone levels were detectable by
species-specific ELISA analysis of plasma samples on day 5 after
transplantationand increased by 2.5 fold over the observation (19
days) in contrst to controls (day 19: 82.5+/-29.3 vs. 0.0+/-0.0
pg/ml; p