Nachweis und Vergleich von Mikro-Ribonukleinsäure Expressionsprofilen Serum von gesunden und an der dilatativen Kardiomyopathie erkrankten Dobermännern
Beschreibung
vor 10 Jahren
Background: Dilated cardiomyopathy (DCM) is the most common heart
disease in Doberman Pinschers. MicroRNAs (miRNAs) are short
non-coding RNAs playing important roles in gene regulation.
Different miRNA expression patterns have been described for DCM in
humans and might represent potential diagnostic markers. There are
no studies investigating miRNA expression profiles in canine DCM.
The aims of this study were to Screen the miRNA expression profile
of canine serum using miRNA microarray and to compare expression
patterns of a group of Doberman Pinschers with DCM and healthy
controls. Results: Eight Doberman Pinschers were examined by
echocardiography and 24-hour-ECG and classified as healthy (n = 4)
or suffering from DCM (n = 4). Total RNA was extracted from serum
and hybridized on a custom-designed 8x60k miRNA microarray
(Agilent) containing probes for 1368 individual miRNAs. Although
total RNA concentrations were very low in serum samples, 404
different miRNAs were detectable with sufficient signal intensity
on miRNA microarray. 22 miRNAs were differentially expressed in the
two groups (p < 0.05 and fold change (FC) > 1.5), but did not
reach statistical significance after multiple testing correction
(false discovery rate adjusted p > 0.05). Five miRNAs were
selected for further analysis using quantitative Real-Time RT-PCR
(qPCR) assays. No significant differences were found using specific
miRNA qPCR assays (p > 0.05). Conclusions: Numerous miRNAs can
be detected in canine serum. Between healthy and DCM dogs, miRNA
expression changes could be detected, but the results did not reach
statistical significance most probably due to the small group size.
miRNAs are potential new circulating biomarkers in veterinary
medicine and should be investigated in larger patient groups and
additional canine diseases.
disease in Doberman Pinschers. MicroRNAs (miRNAs) are short
non-coding RNAs playing important roles in gene regulation.
Different miRNA expression patterns have been described for DCM in
humans and might represent potential diagnostic markers. There are
no studies investigating miRNA expression profiles in canine DCM.
The aims of this study were to Screen the miRNA expression profile
of canine serum using miRNA microarray and to compare expression
patterns of a group of Doberman Pinschers with DCM and healthy
controls. Results: Eight Doberman Pinschers were examined by
echocardiography and 24-hour-ECG and classified as healthy (n = 4)
or suffering from DCM (n = 4). Total RNA was extracted from serum
and hybridized on a custom-designed 8x60k miRNA microarray
(Agilent) containing probes for 1368 individual miRNAs. Although
total RNA concentrations were very low in serum samples, 404
different miRNAs were detectable with sufficient signal intensity
on miRNA microarray. 22 miRNAs were differentially expressed in the
two groups (p < 0.05 and fold change (FC) > 1.5), but did not
reach statistical significance after multiple testing correction
(false discovery rate adjusted p > 0.05). Five miRNAs were
selected for further analysis using quantitative Real-Time RT-PCR
(qPCR) assays. No significant differences were found using specific
miRNA qPCR assays (p > 0.05). Conclusions: Numerous miRNAs can
be detected in canine serum. Between healthy and DCM dogs, miRNA
expression changes could be detected, but the results did not reach
statistical significance most probably due to the small group size.
miRNAs are potential new circulating biomarkers in veterinary
medicine and should be investigated in larger patient groups and
additional canine diseases.
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