Vergleichende Differenzierung von Clostridium spp. mittels biochemischer und molekularbiologischer Methoden sowie MALDI-TOF Massenspektrometrie
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vor 11 Jahren
The aim of this study was to examine whether the classical
biochemical differentiation methods in relation to clostridial
analysis can be replaced by newer molecular biological techniques
such as real-time PCR and by MALDI-TOF MS. For this purpose, 118
potentially pathogenic Clostridium spp. isolates were compared at
the Bavarian Health and Food Safety Authority using the above
mentioned methods. On the basis of the obtained results a
recommendation concerning the general approach to the detection of
Clostridium spp. in the food industry has been elaborated.
biochemical differentiation methods in relation to clostridial
analysis can be replaced by newer molecular biological techniques
such as real-time PCR and by MALDI-TOF MS. For this purpose, 118
potentially pathogenic Clostridium spp. isolates were compared at
the Bavarian Health and Food Safety Authority using the above
mentioned methods. On the basis of the obtained results a
recommendation concerning the general approach to the detection of
Clostridium spp. in the food industry has been elaborated.
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