Etablierung eines humanen Modells zur Untersuchung des akuten Ischämieschmerzes am Beispiel von Adenosin

Purpose The aim of this study was to establish a method to examine
acute ischemic pain in humans using the example of adenosine.
Materials and Methods 11 patients with unilateral peripheral
arterial disease (PAD) performed a treadmill exercise until
intolerable exercise-induced ischemic pain. Blood gas analysis
(BGA) and blood samples were taken before treadmill examination
(baseline), directly after treadmill examination and after
recovery. The ankle-brachial index (ABI) and systemic blood
pressure was measured at the same time. The ABI was measured via
conventional Doppler method. High-performance liquid chromatography
(HPLC) was used to determine the purine adenosine and its
metabolite inosine. S100B-serum levels were detected via Elecsys
S100B Immunoassay (Roche Diagnostics, Penzberg Deutschland).
Results Treadmill exercise lead to intolerable exercise-induced
ischemic pain in the PAD affected leg. ABI decreased to
pathological values. BGA and blood samples showed typical ischemic
changes. A systemic increase in adenosine levels was measured,
whereas no local increase in the PAD affected leg could be found. A
correlation between S100B and peripheral nerve damage could not be
found. Conclusion The established method is an effective model to
examine acute ischemic pain in humans.